Overview

  • Product nameZinc Quantification Kit
    See all Zinc kits
  • Detection methodColorimetric
  • Tests
    100 x 1 assay
  • Sample type
    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay typeQuantitative
  • Sensitivity
    > 1 µM
  • Range
    1 µM - 3 µM
  • Assay time
    0h 15m
  • Assay durationMultiple steps standard assay
  • Product overview

    Abcam's Zinc Quantification Kit is a convenient colorimetric assay in which Zinc binds to a ligand with development of absorbance at 560 nm. The assay can be used with biological samples such as serum, plasma, csf or urine with detection sensitivity 0.2 µg/ml (~1-3 µM).
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Zinc, a metallic chemical element, symbol Zn and atomic number 30 is chemically similar to Magnesium due to its similar size and sole oxidation state of 2+. Zinc is an essential mineral of great biological significance, because many enzymes require it as an essential cofactor. Examples of zinc's biological roles include signal transduction, gene expression, regulation of apoptosis, synaptic plasticity and prostate gland function.

  • Tested applicationsSuitable for: Functional Studiesmore details
  • PlatformMicroplate

Properties

  • RelevanceZinc, a metallic chemical element, symbol Zn and atomic number 30 is chemically similar to Magnesium due to its similar size and sole oxidation state of +2. Zinc is an essential mineral of great biological significance since many enzymes require it as an essential cofactor. Examples of zinc’s biological roles include signal transduction, gene expression, regulation of apoptosis, synaptic plasticity and prostate gland function.
  • Alternative names
    • Zn
    • Zn++
    • Zn2+

Applications

Our Abpromise guarantee covers the use of ab102507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Zinc Quantification Kit images

  • Zinc measured in biological fluids showing quantity (nmol) per microliter of tested sample

  • Zinc measured in cell lysates showing quantity (nmol) per 1 mln of tested cells

  • Zinc measured in mouse tissue lysates showing quantity (nmol) per mg protein of tested sample

  • Standard curve (colourimetric) : mean of duplicates (+/- SD) with background substracted

Protocols

References for Zinc Quantification Kit (ab102507)

ab102507 has not yet been referenced specifically in any publications.

Product Wall

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I heard an update from a different member of our lab and they said thatthe correlation between Zinc concentration determined using Abcam method and reference method such as Atomic Absorption Spectr...

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You have kindly asked in your last question "I need to quantify ZnSO4 in aqueous samples and I would like to know if the Kit is suitable also for the salt detection and not only for the ion Zn2+." My answ...

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The ab102507 kit based on the chemistry of exchange of electrons between ligand and Zn2+ ions. The ligand is oxidized with interaction of Zn2+ ions with the development of colour which has absorbance maxima at 560n...

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Do you have more information about the range of wavelengths that are read when your plate reader is set to 590 nm? Most plate readers have flexibility in their band width of detection, which allows them to get the readout in a range of wavele...

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To calculate the concentration of Zn

Zn = Sa (is the sample amount from standard curve corresponding to the OD of sample) / volume of the samples (in your case it will be 20ul, if you start with 20 and 50 ...

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I apologize for this mistake. The lysis buffer is a standard lysis buffer that can be used for our kits, but you are correct, in this case EDTA should not be added.

Please omit EDTA when preparing the lysis...

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I am happy to confirm that ab102507 can be used for mammalian cells. Please find the recommended lysis buffer for mammalian cells below:

Solutions and Reagents:

Note: Prepare solutions with Mill...

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The correlation experiment was done as a part of an external study and unfortunately the lab told me that further details were unavailable. However, they did say that 40 samples were compared.

I hope this i...

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Thank you for your reply. Below you may find the answers to your questions.


1. What is the linear range of the assay? 0 - 306 uM (or 0 - 20 ug/ml)


2. What is Limit of Detection? ˜ 0.61 uM (or 0.04 ug/ml)

3. Wh...

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I heard back from the lab regarding your questions. Please find the answers below:

1) Zinc is a pure chemical source: ZnCl2
2) Other divalents Copper, Cobalt are neutralized by a masking agent,...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"