Overview

  • Product nameAnti-ZIP Kinase antibody
    See all ZIP Kinase primary antibodies
  • Description
    Rabbit polyclonal to ZIP Kinase
  • Tested applicationsSuitable for: WB, IHC-P, ICCmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human ZIP Kinase (internal sequence).
    Database link: O43293

  • Positive control
    • Human small intestine and Human brain cortex tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab115695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 53 kDa.
IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC Use at an assay dependent concentration.

Target

  • FunctionSerine/threonine kinase which acts as a positive regulator of apoptosis. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. Regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. Involved in the formation of promyelocytic leukemia protein nuclear body (PML-NB), one of many subnuclear domains in the eukaryotic cell nucleus, and which is involved in oncogenesis and viral infection.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Ubiquitinated. Ubiquitination mediated by the UBE2D3 E3 ligase does not lead to proteasomal degradation, but influences promyelocytic leukemia protein nuclear bodies (PML-NBs) formation in the nucleus.
    Autophosphorylated. Phosphorylated by ROCK1.
  • Cellular localizationNucleus. Cytoplasm. Nucleus > PML body. Relocates to the cytoplasm on binding PAWR where the complex appears to interact with actin filaments (By similarity). Localizes to promyelocytic leukemia protein nuclear bodies (PML-NBs). Associates to centromeres from prophase to anaphase.
  • Information by UniProt
  • Database links
  • Alternative names
    • DAP kinase 3 antibody
    • DAP like kinase antibody
    • DAP-like kinase antibody
    • Dapk 3 antibody
    • DAPK3 antibody
    • DAPK3_HUMAN antibody
    • Death associated kinase 3 antibody
    • Death associated protein kinase 3 antibody
    • Death-associated protein kinase 3 antibody
    • Dlk antibody
    • EC 2.7.11.1 antibody
    • FLJ36473 antibody
    • MYPT1 kinase antibody
    • ZIP antibody
    • ZIP kinase antibody
    • ZIP kinase isoform antibody
    • ZIP-kinase antibody
    • ZIPK antibody
    • zipper-interacting protein kinase antibody
    see all

Anti-ZIP Kinase antibody images

  • Immunohistochemistry Image of Human Small Intestine, Peyer's Patch (formalin-fixed, paraffin-embedded) stained with ZIP kinase antibody (ab115695) at 2.5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Immunohistochemistry Image of Human Brain, Cortex (formalin-fixed, paraffin-embedded) stained with ZIP kinase antibody (ab115695) at 2.5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References for Anti-ZIP Kinase antibody (ab115695)

ab115695 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Permeabilization Yes - 0.5% TritonX100 in PBS
Specification HeLa
Fixative Paraformaldehyde
Username

Dr. Kirk McManus

Verified customer

Submitted Jul 22 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"