Overview

  • Product nameAnti-ZIP Kinase antibody
    See all ZIP Kinase primary antibodies
  • Description
    Rabbit polyclonal to ZIP Kinase
  • SpecificityIt has no cross reactivity to DAP kinase.
  • Tested applicationsSuitable for: ELISA, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Other Immunogen Type corresponding to ZIP Kinase. ZIP kinase antibody was raised against a peptide corresponding to amino acids near the center of human ZIP kinase.
    Sequence:

    RRRNVRGEDSGRKPERRRLK


    (Peptide available as ab8383)

  • Positive control
    • HeLa whole cell lysate or Jurkat whole cell lysate.
  • General notesApoptosis inducing kinase.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab2057 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
ICC/IF Use a concentration of 10 µg/ml.
WB Use at an assay dependent concentration. Can be blocked with ZIP Kinase peptide (ab8383).

Target

  • FunctionSerine/threonine kinase which acts as a positive regulator of apoptosis. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. Regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. Involved in the formation of promyelocytic leukemia protein nuclear body (PML-NB), one of many subnuclear domains in the eukaryotic cell nucleus, and which is involved in oncogenesis and viral infection.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Ubiquitinated. Ubiquitination mediated by the UBE2D3 E3 ligase does not lead to proteasomal degradation, but influences promyelocytic leukemia protein nuclear bodies (PML-NBs) formation in the nucleus.
    Autophosphorylated. Phosphorylated by ROCK1.
  • Cellular localizationNucleus. Cytoplasm. Nucleus > PML body. Relocates to the cytoplasm on binding PAWR where the complex appears to interact with actin filaments (By similarity). Localizes to promyelocytic leukemia protein nuclear bodies (PML-NBs). Associates to centromeres from prophase to anaphase.
  • Information by UniProt
  • Database links
  • Alternative names
    • DAP kinase 3 antibody
    • DAP like kinase antibody
    • DAP-like kinase antibody
    • Dapk 3 antibody
    • DAPK3 antibody
    • DAPK3_HUMAN antibody
    • Death associated kinase 3 antibody
    • Death associated protein kinase 3 antibody
    • Death-associated protein kinase 3 antibody
    • Dlk antibody
    • EC 2.7.11.1 antibody
    • FLJ36473 antibody
    • MYPT1 kinase antibody
    • ZIP antibody
    • ZIP kinase antibody
    • ZIP kinase isoform antibody
    • ZIP-kinase antibody
    • ZIPK antibody
    • zipper-interacting protein kinase antibody
    see all

Anti-ZIP Kinase antibody images

  • All lanes : Anti-ZIP Kinase antibody (ab2057) at 1/500 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : Jurkat whole cell lysate


    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  • ab2057 at 10µg/ml staining of ZIP kinase in Jurkat cell by ICC/IF

  • Immunofluorescence of ZIPK in Jurkat cells using ab2057 at 10 ug/ml.

References for Anti-ZIP Kinase antibody (ab2057)

This product has been referenced in:
  • Puto LA & Reed JC Daxx represses RelB target promoters via DNA methyltransferase recruitment and DNA hypermethylation. Genes Dev 22:998-1010 (2008). Read more (PubMed: 18413714) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (Heart)
Gel Running Conditions Reduced Denaturing (12% acrylamide)
Loading amount 20 µg
Specification Heart
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jul 27 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"