Overview

  • Product name
  • Description
    Rabbit polyclonal to ZIP Kinase
  • Specificity
    It has no cross reactivity to DAP kinase.
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human ZIP Kinase aa 279-298 (internal sequence). ZIP kinase antibody was raised against a peptide corresponding to amino acids near the center of human ZIP kinase.
    Sequence:

    RRRNVRGEDSGRKPERRRLK


    Database link: O43293
    (Peptide available as ab8383)

  • Positive control
    • HeLa whole cell lysate or Jurkat whole cell lysate.
  • General notes
    Apoptosis inducing kinase.

Properties

Applications

Our Abpromise guarantee covers the use of ab2057 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB Use at an assay dependent concentration. Can be blocked with Human ZIP Kinase peptide (ab8383).

Target

  • Function
    Serine/threonine kinase which acts as a positive regulator of apoptosis. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. Regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. Involved in the formation of promyelocytic leukemia protein nuclear body (PML-NB), one of many subnuclear domains in the eukaryotic cell nucleus, and which is involved in oncogenesis and viral infection.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Ubiquitinated. Ubiquitination mediated by the UBE2D3 E3 ligase does not lead to proteasomal degradation, but influences promyelocytic leukemia protein nuclear bodies (PML-NBs) formation in the nucleus.
    Autophosphorylated. Phosphorylated by ROCK1.
  • Cellular localization
    Nucleus. Cytoplasm. Nucleus > PML body. Relocates to the cytoplasm on binding PAWR where the complex appears to interact with actin filaments (By similarity). Localizes to promyelocytic leukemia protein nuclear bodies (PML-NBs). Associates to centromeres from prophase to anaphase.
  • Information by UniProt
  • Database links
  • Alternative names
    • DAP kinase 3 antibody
    • DAP like kinase antibody
    • DAP-like kinase antibody
    • Dapk 3 antibody
    • DAPK3 antibody
    • DAPK3_HUMAN antibody
    • Death associated kinase 3 antibody
    • Death associated protein kinase 3 antibody
    • Death-associated protein kinase 3 antibody
    • Dlk antibody
    • EC 2.7.11.1 antibody
    • FLJ36473 antibody
    • MYPT1 kinase antibody
    • ZIP antibody
    • ZIP kinase antibody
    • ZIP kinase isoform antibody
    • ZIP-kinase antibody
    • ZIPK antibody
    • zipper-interacting protein kinase antibody
    see all

Images

  • Western Blot of HeLa and Jurkat cell lysates labeling ZIP Kinase with Anti-ZIP Kinase antibody (ab2057) at 1µg/ml.
  • All lanes : Anti-ZIP Kinase antibody (ab2057) at 1/500 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : Jurkat whole cell lysate


    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  • ab2057 at 10µg/ml staining of ZIP kinase in Jurkat cell by ICC/IF

  • Immunofluorescence of ZIPK in Jurkat cells using ab2057 at 10 ug/ml.

References

This product has been referenced in:
  • Puto LA & Reed JC Daxx represses RelB target promoters via DNA methyltransferase recruitment and DNA hypermethylation. Genes Dev 22:998-1010 (2008). Read more (PubMed: 18413714) »
  • Oster B  et al. Human herpesvirus 6B induces phosphorylation of p53 in its regulatory domain by a CK2- and p38-independent pathway. J Gen Virol 89:87-96 (2008). Read more (PubMed: 18089732) »

See all 2 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Heart)
Gel Running Conditions
Reduced Denaturing (12% acrylamide)
Loading amount
20 µg
Specification
Heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jul 27 2015

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