Synthetic peptide corresponding to Human ZIP Kinase aa 1-100 (N terminal).
Database link: O43293
This product is a recombinant rabbit monoclonal antibody.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Our Abpromise guarantee covers the use of ab79422 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/10000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).|
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: DAPK3 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: A431 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab79422 observed at 53 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab79422 was shown to specifically react with DAPK3 in wild-type HAP1 cells along with other cross-reactive bands. No bands were observed when DAPK3 knockout samples were examined. Wild-type and DAPK3 knockout samples were subjected to SDS-PAGE. Ab79422 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/10,000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
ab79422 has not yet been referenced specifically in any publications.