Anti-ZIP Kinase antibody [EPR1635] (ab79422)

Overview

  • Product nameAnti-ZIP Kinase antibody [EPR1635]
    See all ZIP Kinase primary antibodies
  • Description
    Rabbit monoclonal [EPR1635] to ZIP Kinase
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human ZIP Kinase aa 1-100 (N terminal).
    Database link: O43293

  • Positive control
    • HeLa and A431 cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab79422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/10000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
  • Application notesIs unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • FunctionSerine/threonine kinase which acts as a positive regulator of apoptosis. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. Regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. Involved in the formation of promyelocytic leukemia protein nuclear body (PML-NB), one of many subnuclear domains in the eukaryotic cell nucleus, and which is involved in oncogenesis and viral infection.
    • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Ubiquitinated. Ubiquitination mediated by the UBE2D3 E3 ligase does not lead to proteasomal degradation, but influences promyelocytic leukemia protein nuclear bodies (PML-NBs) formation in the nucleus.
      Autophosphorylated. Phosphorylated by ROCK1.
    • Cellular localizationNucleus. Cytoplasm. Nucleus > PML body. Relocates to the cytoplasm on binding PAWR where the complex appears to interact with actin filaments (By similarity). Localizes to promyelocytic leukemia protein nuclear bodies (PML-NBs). Associates to centromeres from prophase to anaphase.
    • Information by UniProt
    • Database links
    • Alternative names
      • DAP kinase 3 antibody
      • DAP like kinase antibody
      • DAP-like kinase antibody
      • Dapk 3 antibody
      • DAPK3 antibody
      • DAPK3_HUMAN antibody
      • Death associated kinase 3 antibody
      • Death associated protein kinase 3 antibody
      • Death-associated protein kinase 3 antibody
      • Dlk antibody
      • EC 2.7.11.1 antibody
      • FLJ36473 antibody
      • MYPT1 kinase antibody
      • ZIP antibody
      • ZIP kinase antibody
      • ZIP kinase isoform antibody
      • ZIP-kinase antibody
      • ZIPK antibody
      • zipper-interacting protein kinase antibody
      see all

    Anti-ZIP Kinase antibody [EPR1635] images



    • Predicted band size : 53 kDa

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: DAPK3 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)
      Lane 4: A431 whole cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab79422 observed at 53 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab79422 was shown to specifically react with DAPK3 when DAPK3 knockout samples were used. Wild-type and DAPK3 knockout samples were subjected to SDS-PAGE. Ab79422 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 5000 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-ZIP Kinase antibody [EPR1635] (ab79422) at 1/5000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 53 kDa
      Observed band size : 53 kDa

    References for Anti-ZIP Kinase antibody [EPR1635] (ab79422)

    ab79422 has not yet been referenced specifically in any publications.

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