Validated using a knockout cell line
Recombinant
RabMAb

Anti-ZIP Kinase antibody [EPR1636Y] (ab51602)

Overview

  • Product name
    Anti-ZIP Kinase antibody [EPR1636Y]
    See all ZIP Kinase primary antibodies
  • Description
    Rabbit monoclonal [EPR1636Y] to ZIP Kinase
  • Specificity
    ab51602 recognizes ZIP Kinase.
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human ZIP Kinase aa 400 to the C-terminus (C terminal).
    Database link: O43293

  • Positive control
    • HeLa cell lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab51602 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
Flow Cyt 1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Function
      Serine/threonine kinase which acts as a positive regulator of apoptosis. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. Regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. Involved in the formation of promyelocytic leukemia protein nuclear body (PML-NB), one of many subnuclear domains in the eukaryotic cell nucleus, and which is involved in oncogenesis and viral infection.
    • Sequence similarities
      Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Ubiquitinated. Ubiquitination mediated by the UBE2D3 E3 ligase does not lead to proteasomal degradation, but influences promyelocytic leukemia protein nuclear bodies (PML-NBs) formation in the nucleus.
      Autophosphorylated. Phosphorylated by ROCK1.
    • Cellular localization
      Nucleus. Cytoplasm. Nucleus > PML body. Relocates to the cytoplasm on binding PAWR where the complex appears to interact with actin filaments (By similarity). Localizes to promyelocytic leukemia protein nuclear bodies (PML-NBs). Associates to centromeres from prophase to anaphase.
    • Information by UniProt
    • Database links
    • Alternative names
      • DAP kinase 3 antibody
      • DAP like kinase antibody
      • DAP-like kinase antibody
      • Dapk 3 antibody
      • DAPK3 antibody
      • DAPK3_HUMAN antibody
      • Death associated kinase 3 antibody
      • Death associated protein kinase 3 antibody
      • Death-associated protein kinase 3 antibody
      • Dlk antibody
      • EC 2.7.11.1 antibody
      • FLJ36473 antibody
      • MYPT1 kinase antibody
      • ZIP antibody
      • ZIP kinase antibody
      • ZIP kinase isoform antibody
      • ZIP-kinase antibody
      • ZIPK antibody
      • zipper-interacting protein kinase antibody
      see all

    Images



    • Predicted band size : 53 kDa

      Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: ZIP Kinase knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)
      Lane 4: A431 whole cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab51602 observed at 53 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab51602 was shown to specifically react with ZIP Kinase in wild-type HAP1 cells. No band was observed when ZIP Kinase knockout samples were examined. Wild-type and ZIP Kinase knockout samples were subjected to SDS-PAGE. Ab51602 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    • Anti-ZIP Kinase antibody [EPR1636Y] (ab51602) at 1/1000 dilution + HeLa cell lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 53 kDa
      Observed band size : 53 kDa

    References

    This product has been referenced in:
    • Kocher BA  et al. DAPK3 suppresses acini morphogenesis and is required for mouse development. Mol Cancer Res 13:358-67 (2015). WB ; Human . Read more (PubMed: 25304685) »
    • Marina O  et al. Serologic markers of effective tumor immunity against chronic lymphocytic leukemia include nonmutated B-cell antigens. Cancer Res 70:1344-55 (2010). WB ; Human . Read more (PubMed: 20124481) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Heart)
    Gel Running Conditions
    Reduced Denaturing (12% acrylamide)
    Loading amount
    25 µg
    Specification
    Heart
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted May 11 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up