Anti-ZKSCAN3 antibody (ab187866)
Key features and details
- Rabbit polyclonal to ZKSCAN3
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ZKSCAN3 antibody
See all ZKSCAN3 primary antibodies -
Description
Rabbit polyclonal to ZKSCAN3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human ZKSCAN3 aa 150-300.
Database link: Q9BRR0 -
Positive control
- Human small intestine tissue; Human cell A-431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab187866 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 1 - 4 µg/ml.
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Notes |
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 1 - 4 µg/ml. |
Target
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Function
Acts as a transcriptional regulator. Binds to the consensus sequence 5'-[GT][AG][AGT]GGGG-3'. Associates with chromatin at the ITGB4 and VEGF promoters. Activates the transcription of genes associated with colon cancer progression. -
Tissue specificity
Expressed in colorectal cancers (at protein level). -
Sequence similarities
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 7 C2H2-type zinc fingers.
Contains 1 KRAB domain.
Contains 1 SCAN box domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 80317 Human
- Omim: 612791 Human
- SwissProt: Q9BRR0 Human
- Unigene: 485004 Human
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Alternative names
- dJ874C20.1 antibody
- mszf35 antibody
- SCAN-KRAB-zinc finger protein antibody
see all
Images
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Immunohistochemical analysis of human small intestine tissue labelled for ZKSCAN3 using ab187866. Strong positive nuclear staining observed in glandular cells.
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Immunohistochemical analysis of human placenta tissue labelled for ZKSCAN3 using ab187866. Positive nuclear and cytoplasmic staining observed in trophoblastic cells.
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Immunohistochemical analysis of human cervical cancer tissue labelled for ZKSCAN3 using ab187866. Positive nuclear staining observed in tumor cells.
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Immunohistochemical analysis of human tonsil tissue labelled for ZKSCAN3 using ab187866. Positive nuclear staining observed in germinal and non-germinal center cells.
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Immunohistochemical analysis of human prostate tissue labelled for ZKSCAN3 using ab187866. Positive nuclear and cytoplasmic staining observed in glandular cells.
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Immunofluorescent analysis of Human cell line A-431 labeling ZKSCAN3 with ab187866 at 4µg/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab187866 has been referenced in 1 publication.
- Takano Y et al. Prognostic Significance of ZKSCAN3 (ZNF306) Expression in Gastric Carcinoma. Anticancer Res 40:81-86 (2020). PubMed: 31892555