Recombinant
RabMAb

Anti-Zmat2 antibody [EPR11136] (ab169541)

Overview

  • Product name
    Anti-Zmat2 antibody [EPR11136]
  • Description
    Rabbit monoclonal [EPR11136] to Zmat2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human Zmat2 (UniProt: Q96NC0).

  • Positive control
    • Jurkat, U87-MG, Ramos, HeLa and SH-SY5Y cell lysates; Human colon tissue and ovarian carcinoma tissue; HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR11136
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab169541 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    Images

    • All lanes : Anti-Zmat2 antibody [EPR11136] (ab169541) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : U87-MG cell lysat
      Lane 3 : Ramos cell lysate
      Lane 4 : HeLa cell lysate
      Lane 5 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 24 kDa
      Observed band size: 24 kDa

    • Immunohistochemical analysis of paraffin embedded Human colon tissue labeling Zmat2 with ab169541 antibody at 1/50.
    • Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue labeling Zmat2 with ab169541 antibody at 1/50.
    • Immunofluorescent analysis of HeLa cells labeling Zmat2 with ab169541 at 1/250.

    References

    ab169541 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab169541.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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