Overview

  • Product nameAnti-ZNF133 antibody
    See all ZNF133 primary antibodies
  • Description
    Mouse monoclonal to ZNF133
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 121-231 of human ZNF133 (NP_003425) with a 26 kDa tag.

  • Positive control
    • Hela and SJCRH30 cell lysates

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab77461 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 73 kDa.
ELISA Use at an assay dependent dilution. Detection limit for this product is ~30 ng/ml when used as a capture antibody.

Target

  • FunctionMay be involved in transcriptional regulation as a repressor.
  • Tissue specificitySeems ubiquitous. Seen in the heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 15 C2H2-type zinc fingers.
    Contains 1 KRAB domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • pHZ 13 antibody
    • pHZ 66 antibody
    • Zinc finger protein 133 (clone pHZ13) antibody
    • Zinc finger protein 133 antibody
    • Zinc finger protein 150 (pHZ66) antibody
    • Zinc finger protein 150 antibody
    • ZN133_HUMAN antibody
    • ZNF133 antibody
    • ZNF150 antibody
    see all

Anti-ZNF133 antibody images

  • Anti-ZNF133 antibody (ab77461) at 1/500 dilution + immunogen at 0.2 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 73 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  • Anti-ZNF133 antibody (ab77461) at 1/500 dilution + HeLa cell lysate at 25 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 73 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.
  • Anti-ZNF133 antibody (ab77461) at 1/500 dilution + SJCRH30 cell lysate at 25 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 73 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)

References for Anti-ZNF133 antibody (ab77461)

ab77461 has not yet been referenced specifically in any publications.

Product Wall

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