Overview

  • Product nameAnti-ZNF133 antibody
    See all ZNF133 primary antibodies
  • Description
    Rabbit polyclonal to ZNF133
  • Tested applicationsSuitable for: IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 180-229 (LRGVELEASP AQTGNPEETD KLLKRIEVLG FGTVNCGECG LSFSKMTNLL) of Human ZNF133 (NP_003425).

  • Positive control
    • 721_B cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab85474 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/50.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 73 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionMay be involved in transcriptional regulation as a repressor.
  • Tissue specificitySeems ubiquitous. Seen in the heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 15 C2H2-type zinc fingers.
    Contains 1 KRAB domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • pHZ 13 antibody
    • pHZ 66 antibody
    • Zinc finger protein 133 (clone pHZ13) antibody
    • Zinc finger protein 133 antibody
    • Zinc finger protein 150 (pHZ66) antibody
    • Zinc finger protein 150 antibody
    • ZN133_HUMAN antibody
    • ZNF133 antibody
    • ZNF150 antibody
    see all

Anti-ZNF133 antibody images

  • Anti-ZNF133 antibody (ab85474) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 73 kDa
    Observed band size : 64 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 12%
  • Immunofluorescent image of ab85474 staining ZNF133 in Mouse corneal tissue. a) corneal epithelium b) corneal endothelium c) limbus

References for Anti-ZNF133 antibody (ab85474)

ab85474 has not yet been referenced specifically in any publications.

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