Overview

  • Product name
    Anti-ZNF133 antibody
  • Description
    Rabbit polyclonal to ZNF133
  • Tested applications
    Suitable for: IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within internal amino acids 604-653 (PYVCKTCGRG FSLKSHLSRH RKTTSVHHRL PVQPDPEPCA GQPSDSLYSL) of Human ZNF133 (NP_003425).

  • Positive control
    • THP1 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab94754 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/100.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 73 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    May be involved in transcriptional regulation as a repressor.
  • Tissue specificity
    Seems ubiquitous. Seen in the heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • Sequence similarities
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 15 C2H2-type zinc fingers.
    Contains 1 KRAB domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • pHZ 13 antibody
    • pHZ 66 antibody
    • Zinc finger protein 133 (clone pHZ13) antibody
    • Zinc finger protein 133 antibody
    • Zinc finger protein 150 (pHZ66) antibody
    • Zinc finger protein 150 antibody
    • ZN133_HUMAN antibody
    • ZNF133 antibody
    • ZNF150 antibody
    see all

Images

  • Immunohistochemistry (Frozen sections) labeling ZNF133 with ab94754 at 1:100. Tissue was fixed with cold acetone for 10 minutes. rinsed in phosphate buffered saline (PBS) and incubated with the primary antibody diluted in 1% bovine serum albumin (BSA) in PBS for 1 hour at room temperature.

  • Anti-ZNF133 antibody (ab94754) at 1 µg/ml (in 5% skim milk / PBS buffer) + THP1 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 73 kDa
    Gel concentration: 12%

References

ab94754 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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