Synthetic peptide corresponding to a region within internal amino acids 604-653 (PYVCKTCGRG FSLKSHLSRH RKTTSVHHRL PVQPDPEPCA GQPSDSLYSL) of Human ZNF133 (NP_003425).
Our Abpromise guarantee covers the use of ab94754 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 73 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
Immunohistochemistry (Frozen sections) labeling ZNF133 with ab94754 at 1:100. Tissue was fixed with cold acetone for 10 minutes. rinsed in phosphate buffered saline (PBS) and incubated with the primary antibody diluted in 1% bovine serum albumin (BSA) in PBS for 1 hour at room temperature.
ab94754 has not yet been referenced specifically in any publications.