Overview

  • Product nameAnti-ZNF143 antibody
    See all ZNF143 primary antibodies
  • Description
    Mouse monoclonal to ZNF143
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-627 of Human ZNF143

Properties

Applications

Our Abpromise guarantee covers the use of ab58168 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 68 kDa.
IHC-P Use a concentration of 3 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • RelevanceZNF143 is a human homolog of Xenopus transcriptional activator staf that is involved in selenocystyl tRNA transcription which binds to the SPH motif of small nuclear RNA (snRNA) gene promoters.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • MGC66276 antibody
    • mStaf antibody
    • pHZ 1 antibody
    • SBF antibody
    • Selenocysteine tRNA gene transcription activating factor antibody
    • SPH binding factor antibody
    • STAF antibody
    • Zfp143 antibody
    • zgc:66276 antibody
    • Zinc finger protein 143 antibody
    see all

Anti-ZNF143 antibody images

  • ZNF143 antibody (ab58168) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human ovary, clear cell carcinoma tissue.


  • Predicted band size : 68 kDa
    ZNF143 antibody (ab58168) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ab58168 at 10 ug/ml staining ZNF143 in human Hella cells by Immunocytochemistry/ Immunofluorescence.

References for Anti-ZNF143 antibody (ab58168)

ab58168 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (HEK293T)
Gel Running Conditions Reduced Denaturing (4-10% gel)
Loading amount 30 µg
Treatment siRNA
Specification HEK293T
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Alina Radziwon

Verified customer

Submitted Nov 09 2016

Application Western blot
Sample Human Cell lysate - whole cell (Fibroblasts)
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Loading amount 40 µg
Specification Fibroblasts
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 10 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"