Overview

  • Product name
  • Description
    Mouse monoclonal to ZNF143
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-627 of Human ZNF143

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab58168 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 68 kDa.
IHC-P Use a concentration of 3 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Relevance
    ZNF143 is a human homolog of Xenopus transcriptional activator staf that is involved in selenocystyl tRNA transcription which binds to the SPH motif of small nuclear RNA (snRNA) gene promoters.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • MGC66276 antibody
    • mStaf antibody
    • pHZ 1 antibody
    • SBF antibody
    • Selenocysteine tRNA gene transcription activating factor antibody
    • SPH binding factor antibody
    • STAF antibody
    • Zfp143 antibody
    • zgc:66276 antibody
    • Zinc finger protein 143 antibody
    see all

Anti-ZNF143 antibody images

  • ZNF143 antibody (ab58168) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human ovary, clear cell carcinoma tissue.


  • Predicted band size : 68 kDa
    ZNF143 antibody (ab58168) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ab58168 at 10 ug/ml staining ZNF143 in human Hella cells by Immunocytochemistry/ Immunofluorescence.

References for Anti-ZNF143 antibody (ab58168)

ab58168 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293T)
Gel Running Conditions
Reduced Denaturing (4-10% gel)
Loading amount
30 µg
Treatment
siRNA
Specification
HEK293T
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Alina Radziwon

Verified customer

Submitted Nov 09 2016

Application
Western blot
Sample
Human Cell lysate - whole cell (Fibroblasts)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
40 µg
Specification
Fibroblasts
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 10 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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