Overview

  • Product name
  • Description
    Rabbit polyclonal to ZNF148
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment within Human ZNF148 (N terminal). The exact sequence is proprietary.
    Database link: Q9UQR1

  • Positive control
    • WB: HEK-293T whole cell lysate. IHC-P: Human breast cancer tissue. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab227076 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 89 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Involved in transcriptional regulation. Represses the transcription of a number of genes including gastrin, stromelysin and enolase. Binds to the G-rich box in the enhancer region of these genes.
  • Sequence similarities
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 4 C2H2-type zinc fingers.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Transcription factor ZBP-89 antibody
    • Transcription factor ZBP89 antibody
    • ZBP89 antibody
    • Zinc finger DNA binding protein 89 antibody
    • Zinc finger DNA-binding protein 89 antibody
    • Zinc finger protein 148 antibody
    • ZN148_HUMAN antibody
    • ZNF 148 antibody
    • ZNF148 antibody
    see all

Images

  • Anti-ZNF148 antibody (ab227076) at 1/1000 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

    Developed using the ECL technique.

    Predicted band size: 89 kDa

  • Paraffin-embedded human breast cancer tissue stained for ZNF148 with ab227076 at 1/250 dilution in immunohistochemical analysis.

  • Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ZNF148 (green) using ab227076 at1/500 dilution in ICC/IF.

    Blue: Hoechst 33342 staining.

     

References

ab227076 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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