Overview

  • Product nameAnti-ZNF148 antibody
    See all ZNF148 primary antibodies
  • Description
    Rabbit polyclonal to ZNF148
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide (Human) from an internal region

  • Positive control
    • Human brain tissue HepG2 cell extract

Properties

Applications

Our Abpromise guarantee covers the use of ab69933 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa).
ELISA 1/5000.
IHC-P 1/50 - 1/100.

Target

Anti-ZNF148 antibody images

  • Immunohistochemical analysis of paraffin embedded human brain tissue using ab69933 at 1/50 dilution. Right panel was treated with immunising peptide.
  • All lanes : Anti-ZNF148 antibody (ab69933) at 1/500 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HepG2 cell extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 89 kDa
    Observed band size : 89 kDa
    Additional bands at : 95 kDa. We are unsure as to the identity of these extra bands.

References for Anti-ZNF148 antibody (ab69933)

This product has been referenced in:
  • Zhang W  et al. Upregulation of nemo-like kinase is an independent prognostic factor in colorectal cancer. World J Gastroenterol 21:8836-47 (2015). IHC-P ; Human . Read more (PubMed: 26269673) »
  • Pourfarzad F  et al. Locus-specific proteomics by TChP: targeted chromatin purification. Cell Rep 4:589-600 (2013). WB ; Human . Read more (PubMed: 23911284) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Intestine)
Specification Intestine
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
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Verified customer

Submitted Jul 18 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"