Overview

  • Product name
    Anti-ZNF161 antibody [ZNF5J141]
    See all ZNF161 primary antibodies
  • Description
    Mouse monoclonal [ZNF5J141] to ZNF161
  • Tested applications
    Suitable for: WB, ICC, Dot blotmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant human ZNF161 fragement

  • Positive control
    • HeLa cells and whole cell lysate and NIH3T3 whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab50970 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50. Detects a band of approximately 60 kDa (predicted molecular weight: 57 kDa).
ICC 1/1.
Dot blot Use at an assay dependent dilution.

Target

  • Function
    Possible transcription factor. Specifically binds to the CT/GC-rich region of the interleukin-3 promoter and mediates tax transactivation of IL-3.
  • Tissue specificity
    Ubiquitously expressed. Highest levels in skeletal muscle and kidney.
  • Sequence similarities
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 6 C2H2-type zinc fingers.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DB1 antibody
    • MGC82370 antibody
    • Putative transcription factor DB1 antibody
    • Vascular endothelial zinc finger 1 antibody
    • VEZF1 antibody
    • VEZF1_HUMAN antibody
    • Zinc finger protein 161 antibody
    • ZNF161 antibody
    see all

Images

  • Anti-ZNF161 antibody [ZNF5J141] (ab50970) at 1/50 dilution + HeLa whole cell lysate at 50 µg

    Secondary
    anti-mouse IgG at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 57 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • ab50970 at 1/1 dilution staining human ZNF161 in HeLa cells by Immunocytochemistry. AlexaFluor 488 Goat Anti-mouse IgG at 1/200 as secondary.
  • Anti-ZNF161 antibody [ZNF5J141] (ab50970) at 1/50 dilution + NIH3T3 whole cell lysate at 50 µg

    Secondary
    anti-mouse IgG at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 57 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Gerald D  et al. RhoB controls coordination of adult angiogenesis and lymphangiogenesis following injury by regulating VEZF1-mediated transcription. Nat Commun 4:2824 (2013). WB . Read more (PubMed: 24280686) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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