The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesWB: Use at a concentration of 1-5 µg/ml. IF/ICC: Use at a concentration of 10 µg/ml.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Tissue specificityExpressed in a variety of organs, but most strongly in adult testis and ovary followed by small intestine, colon, prostate, thymus, spleen, pancreas, skeletal muscle, heart, brain and kidney. Also expressed in umbilical vein endothelial cells, foreskin fibroblast and HEPG2 cells.
Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family. Contains 3 C2H2-type zinc fingers. Contains 1 SCAN box domain.