Overview

  • Product name
  • Description
    Rabbit polyclonal to ZNF185
  • Specificity
    ab83100 recognizes ZNF185 variant 689.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within residues corresponding to N terminal amino acids 144-193 (LAPYNIRRSS TSGDTEEEEE EEVVPFSSDE QKRRSEAASG VLRRTAPREH) of human ZNF185 (NP_009081).

  • Positive control
    • HeLa cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab83100 is purified by a peptide affinity chromatography method.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab83100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 73 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:12500.

Target

  • Function
    May be involved in the regulation of cellular proliferation and/or differentiation.
  • Tissue specificity
    Expressed in placenta, pancreas and kidney. Also expressed in prostate, testis, ovary and blood.
  • Sequence similarities
    Contains 1 LIM zinc-binding domain.
  • Cellular localization
    Cytoplasm > cytoskeleton. Cell junction > focal adhesion.
  • Information by UniProt
  • Database links
  • Alternative names
    • LIM domain protein ZNF185 antibody
    • P1 A antibody
    • P1-A antibody
    • Zinc finger protein 185 (LIM domain) antibody
    • Zinc finger protein 185 antibody
    • ZN185_HUMAN antibody
    • ZNF185 antibody
    see all

Anti-ZNF185 antibody images

  • Anti-ZNF185 antibody (ab83100) at 1 µg/ml + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 73 kDa
    Observed band size : 73 kDa
    Additional bands at : 24 kDa. We are unsure as to the identity of these extra bands.

References for Anti-ZNF185 antibody (ab83100)

ab83100 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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