• Product nameAnti-ZNF195 antibody
  • Description
    Rabbit polyclonal to ZNF195
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Cow, Dog
  • Immunogen

    A region within synthetic peptide: LTFRDVAIEF SLEEWKCLDL AQQNLYRDVM LENYRNLFSV GLTVCKPGLI, corresponding to amino acids 4-53 of Human ZNF195

  • Positive control
    • WB: Foetal lung lysate IHC-P: Human lung


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab50600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 72 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

IHC-P Use a concentration of 4 - 8 µg/ml.
IP Use at an assay dependent concentration.


  • RelevanceZinc finger proteins are an important class of eukaryotic DNA binding proteins. The zinc finger motif contains spatially conserved cysteines and histidines, which bind a zinc ion. The most common zinc finger motif is the C2H2 type, which was first identified in the Drosophila segmentation gene, Kruppel. The human genome has several hundred Kruppel related zinc finger genes. About one third of these human Kruppel type genes also code for a highly conserved region, the Kruppel associated box (KRAB domain), comprising approximately 75 amino acids found at the N terminal end. The zinc finger gene ZNF195 contains an N terminal KRAB domain and 14 tandemly repeated Kruppel type zinc finger motifs at its C terminus. ZNF195 may be involved in transcriptional regulation. It is expressed in adult heart, brain, placenta, skeletal muscle and pancreas, and in foetal lung, kidney and brain. There is little expression in adult lung, liver and kidney.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • Zinc finger protein 195 antibody
    • ZNFP104 antibody

Anti-ZNF195 antibody images

  • Anti-ZNF195 antibody (ab50600) at 1 µg/ml + foetal lung lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 72 kDa
    Observed band size : ~64 kDa (why is the actual band size different from the predicted?)
  • ab50600 at a concentration of 4-8 µg/ml labelling alveolar cells (labelled with arrows) in paraffin-embedded human lung tissue. Magnification 400X.

References for Anti-ZNF195 antibody (ab50600)

ab50600 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - nuclear (Multiple cell types)
Loading amount 30 µg
Specification Multiple cell types
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Seth Frietze

Verified customer

Submitted Jun 30 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - nuclear (Liver carcinoma cell line HepG2)
Total protein in input 30 µg
Specification Liver carcinoma cell line HepG2
Immuno-precipitation step Protein A/G

Dr. Seth Frietze

Verified customer

Submitted Jun 30 2011