The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
1/1000. Predicted molecular weight: 75 kDa.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/10 - 1/50.
FunctionTranscriptional repressor that binds to elements found predominantly in genes that participate in lipid metabolism. Among its targets are structural components of lipoprotein particles (apolipoproteins AIV, CIII, and E), enzymes involved in lipid processing (lipoprotein lipase, lecithin cholesteryl ester transferase), transporters involved in lipid homeostasis (ABCA1, ABCG1), and several genes involved in processes related to energy metabolism and vascular disease.
Tissue specificityHighly expressed in testis. Also expressed in breast carcinoma cell lines.
Immunofluorescence of MDA-MB231 cells labelling ZNF202 with ab103034. MDA-MB231 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ab103034 (1:25, 1 h at 37℃). Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used as the secondary antibody (1:400, 50 min at 37℃). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃) and nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). ZNF202 immunoreactivity is localized to the nucleus.
Immunoflurescence of HepG2 cells labelling ZNF202 with ab103034. An Alexa Fluor 488-conjugated Goat anti-rabbit IgG (green) was used as the secondary antibody. DAPI was used to stain the cell nuclei (blue).
Flow Cytometry - Anti-ZNF202 antibody (ab103034)
Flow cytometry of HepG2 cells (right histogram) labelling ZNF202 with ab103034 compared to a negative control (left histogram). FITC-conjugated Goat anti-rabbit was used as the secondary antibody.
Western blot - ZNF202 antibody (ab103034)
Anti-ZNF202 antibody (ab103034) at 1/100 dilution + HepG2 cell line lysates at 35 µg