Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab88262 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
IHC-P
ICC/IF
  • Application notesELISA: Use at an assay dependent dilution.
    ICC/IF: Use at a concentration of 10 µg/ml.
    IHC-P: Use at a concentration of 3 µg/ml. Antigen retrieval is not essential but may optimise staining.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 51 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Anti-ZNF207 antibody images

    • Anti-ZNF207 antibody (ab88262) at 1 µg/ml + immunogen at 0.2 µg

      Secondary
      goat anti-mouse IgG at 1/5000 dilution

      Predicted band size : 51 kDa
      Observed band size : 38 kDa (why is the actual band size different from the predicted?)
    • Anti-ZNF207 antibody (ab88262) at 1 µg/ml + HeLa nuclear extracts at 25 µg

      Secondary
      goat anti-mouse IgG at 1/5000 dilution

      Predicted band size : 51 kDa
    • All lanes : Anti-ZNF207 antibody (ab88262) at 1 µg/ml

      Lane 1 : trasnfected 293T cell lysate
      Lane 2 : untrasnfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      goat anti-mouse IgG at 1/5000 dilution

      Predicted band size : 51 kDa
    • ab88262 at 3ug/ml staining ZNF207 in formalin-fixed paraffin-embedded human adrenal gland tissue.
    • ab88262 at 10ug/ml (o/n incubation at 4deg) staining ZNF207 on HeLa cells, followed by a fluorescein-conjugated secondary.

    References for Anti-ZNF207 antibody (ab88262)

    ab88262 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab88262.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"