This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

Overview

Properties

Applications

  • Application notes

    This antibody gave a positive result in ELISA against the immunizing peptide (ab48177).
    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Binds to the promoters of target genes and functions as repressor. Promotes cell proliferation and antagonizes cell death. Promotes phosphorylation of AKT1 at 'Ser-473'.
    • Sequence similarities
      Belongs to the krueppel C2H2-type zinc-finger protein family.
      Contains 7 C2H2-type zinc fingers.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • ZABC 1 antibody
      • ZABC1 antibody
      • Zinc finger protein 217 antibody
      • ZN 217 antibody
      • ZN217 antibody
      • ZN217_HUMAN antibody
      • ZNF 217 antibody
      • ZNF217 antibody
      see all

    Anti-ZNF217 antibody images

    This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

    • Anti-ZNF217 antibody (ab48133) at 1 µg/ml + MCF7 (Human breast adenocarcinoma cell line) Nuclear Lysate at 8 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size : 115 kDa
      Observed band size : 160 kDa (why is the actual band size different from the predicted?)

    References for Anti-ZNF217 antibody (ab48133)

    This product has been referenced in:
    • Baran-Gale J  et al. An integrative transcriptomics approach identifies miR-503 as a candidate master regulator of the estrogen response in MCF-7 breast cancer cells. RNA 22:1592-603 (2016). WB ; Human . Read more (PubMed: 27539783) »
    • Nguyen NT  et al. A functional interplay between ZNF217 and estrogen receptor alpha exists in luminal breast cancers. Mol Oncol 8:1441-57 (2014). Read more (PubMed: 24973012) »

    See all 2 Publications for this product

    Product Wall

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (human embryonic stem cell lines)
    Gel Running Conditions
    Reduced Denaturing (12%)
    Loading amount
    30 µg
    Specification
    human embryonic stem cell lines
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 02 2017

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Human Embryonic stem cell (HES2))
    Permeabilization
    Yes - 0.25% Trition X-100
    Specification
    Human Embryonic stem cell (HES2)
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 37°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Feb 02 2017

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (human embryonic stem cells)
    Specification
    human embryonic stem cells
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 37°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jan 26 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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