Recombinant
RabMAb

Anti-ZNF217 antibody [EPR6362] (ab124927)

Overview

  • Product name
    Anti-ZNF217 antibody [EPR6362]
    See all ZNF217 primary antibodies
  • Description
    Rabbit monoclonal [EPR6362] to ZNF217
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to residues in the C-terminus of Human ZNF217.

  • Positive control
    • MCF7, PC3 and Caco2 cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab124927 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 140 kDa (predicted molecular weight: 115 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function
      Binds to the promoters of target genes and functions as repressor. Promotes cell proliferation and antagonizes cell death. Promotes phosphorylation of AKT1 at 'Ser-473'.
    • Sequence similarities
      Belongs to the krueppel C2H2-type zinc-finger protein family.
      Contains 7 C2H2-type zinc fingers.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • ZABC 1 antibody
      • ZABC1 antibody
      • Zinc finger protein 217 antibody
      • ZN 217 antibody
      • ZN217 antibody
      • ZN217_HUMAN antibody
      • ZNF 217 antibody
      • ZNF217 antibody
      see all

    Images

    • All lanes : Anti-ZNF217 antibody [EPR6362] (ab124927) at 1/10000 dilution

      Lane 1 : MCF7 cell lysates
      Lane 2 : PC3 cell lysates
      Lane 3 : Caco2 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-conjugated goat anti-rabbit at 1/2000 dilution

      Predicted band size : 115 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab124927 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (human ESCs)
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Loading amount
    30 µg
    Specification
    human ESCs
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 25 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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