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- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 2% Sucrose, PBS
Concentration information loading...
- PurityImmunogen affinity purified
- Research areas
- Compatible Secondaries
- Positive Controls
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 0.5 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
||Use at an assay dependent concentration.
- FunctionMay be involved in transcriptional regulation.
- Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
Contains 10 C2H2-type zinc fingers.
Contains 1 KRAB domain.
- Cellular localizationNucleus.
Information by UniProt
- HGNC:13016 antibody
- KRAB A domain antibody
- Zinc finger protein 223 antibody
Anti-ZNF223 antibody images
Western blot - ZNF223 antibody (ab43149)
Anti-ZNF223 antibody (ab43149) at 0.5 µg/ml + Jurkat cell lysate at 10 µg with 5% skimmed milk in PBS
HRP-conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 56 kDa
Observed band size : 56 kDa
Additional bands at : 80 kDa (possible cross reactivity).
Immunohistochemistry (Paraffin-embedded sections) - ZNF223 antibody (ab43149)
ab43149 at 4.0 µg/ml staining human skin (approximately 10µg) parafin embedded with x400 magnification. Positively labelled squamous epithelial cells indicated with arrows.
References for Anti-ZNF223 antibody (ab43149)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"