Overview

  • Product nameAnti-ZNF223 antibody
    See all ZNF223 primary antibodies
  • Description
    Rabbit polyclonal to ZNF223
  • SpecificityThis antibody recognizes ZNF223.
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A region within synthetic peptide: HEGWSCQQIW EEIASDLTRP QDSTIKSSQF FEQGDAHSQV EEGISIMHTG, corresponding to amino acids 95-144 of Human ZNF223

  • Positive control
    • Jurkat cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab43149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionMay be involved in transcriptional regulation.
  • Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 10 C2H2-type zinc fingers.
    Contains 1 KRAB domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • HGNC:13016 antibody
    • KRAB A domain antibody
    • Zinc finger protein 223 antibody
    • Zinc finger protein ZNF223 antibody
    • ZN223_HUMAN antibody
    • ZNF223 antibody
    see all

Anti-ZNF223 antibody images

  • Anti-ZNF223 antibody (ab43149) at 0.5 µg/ml + Jurkat cell lysate at 10 µg with 5% skimmed milk in PBS

    Secondary
    HRP-conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 56 kDa
    Observed band size : 56 kDa
    Additional bands at : 80 kDa (possible cross reactivity).
  • ab43149 at 4.0 µg/ml staining human skin (approximately 10µg) parafin embedded with x400 magnification. Positively labelled squamous epithelial cells indicated with arrows.

References for Anti-ZNF223 antibody (ab43149)

ab43149 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"