Overview

  • Product name
  • Description
    Rabbit polyclonal to ZNF224
  • Specificity
    This antibody is specific for human ZNF224.
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A region within synthetic peptide: QKSSQGNGYK PSFSDVSHFD FHQQLHSGEK SHTCDECGKN FCYISALRIH, corresponding to N terminal amino acids 145-194 of Human ZNF224

  • Positive control
    • Jurkat cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab33650 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1.5625e+006.
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 82 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    May be involved in transcriptional regulation as a transcriptional repressor. The DEPDC1A-ZNF224 complex may play a critical role in bladder carcinogenesis by repressing the transcription of the A20 gene, leading to transport of NF-KB protein into the nucleus, resulting in suppression of apoptosis of bladder cancer cells.
  • Tissue specificity
    Ubiquitous. Mainly expressed in fetal tissues.
  • Sequence similarities
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 18 C2H2-type zinc fingers.
    Contains 1 KRAB domain.
  • Cellular localization
    Nucleus. Colocalizes with DEPDC1A at the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • BMZF 2 antibody
    • BMZF-2 antibody
    • BMZF2 antibody
    • Bone marrow zinc finger 2 antibody
    • KOX22 antibody
    • Zinc finger 2, bone marrow antibody
    • Zinc finger protein 224 antibody
    • Zinc finger protein 233 antibody
    • Zinc finger protein 255 antibody
    • Zinc finger protein 27 (KOX 22) antibody
    • Zinc finger protein 27 antibody
    • Zinc finger protein KOX22 antibody
    • Zinc finger protein ZNF255 antibody
    • ZN224_HUMAN antibody
    • ZNF224 antibody
    • ZNF255 antibody
    • ZNF27 antibody
    see all

Images

  • Anti-ZNF224 antibody (ab33650) at 2.5 µg/ml + Jurkat cell lysate. at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG. at 1/50000 dilution

    Predicted band size : 82 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Harada Y  et al. Cell-permeable peptide DEPDC1-ZNF224 interferes with transcriptional repression and oncogenicity in bladder cancer cells. Cancer Res 70:5829-39 (2010). WB ; Human . Read more (PubMed: 20587513) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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