Overview

  • Product nameAnti-ZNF225 antibody
    See all ZNF225 primary antibodies
  • Description
    Rabbit polyclonal to ZNF225
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A region within synthetic peptide: SSDLTRFQDS MVNSFQFSKQ DDMPCQVDAG LSIIHVKTET SEGRTCKKSF, corresponding to N terminal amino acids 108-157 of Human ZNF225

  • Positive control
    • HepG2 cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab66372 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3125 µg/ml. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

Target

  • FunctionMay be involved in transcriptional regulation.
  • Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 18 C2H2-type zinc fingers.
    Contains 1 KRAB domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • C2 H2 type zinc finger protein antibody
    • Zinc finger protein 225 antibody
    • ZN225_HUMAN antibody
    • ZNF225 antibody

Anti-ZNF225 antibody images

  • Anti-ZNF225 antibody (ab66372) at 0.3125 µg/ml + HepG2 cell lysate at 10 µg with 5% skim milk / PBS buffer

    Secondary
    HRP conjugated anti-Rabbit IgGat 1/50,000 - 1/100,000 dilution

    Predicted band size : 82 kDa
    Observed band size : 82 kDa
    Additional bands at : 46 kDa. We are unsure as to the identity of these extra bands.

References for Anti-ZNF225 antibody (ab66372)

ab66372 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab66372.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"