Overview

  • Product name
  • Description
    Rabbit polyclonal to ZNF225
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A region within synthetic peptide: SSDLTRFQDS MVNSFQFSKQ DDMPCQVDAG LSIIHVKTET SEGRTCKKSF, corresponding to N terminal amino acids 108-157 of Human ZNF225

  • Positive control
    • HepG2 cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab66372 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3125 µg/ml. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

Target

  • Function
    May be involved in transcriptional regulation.
  • Sequence similarities
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 18 C2H2-type zinc fingers.
    Contains 1 KRAB domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • C2 H2 type zinc finger protein antibody
    • Zinc finger protein 225 antibody
    • ZN225_HUMAN antibody
    • ZNF225 antibody

Images

  • Anti-ZNF225 antibody (ab66372) at 0.3125 µg/ml + HepG2 cell lysate at 10 µg with 5% skim milk / PBS buffer

    Secondary
    HRP conjugated anti-Rabbit IgGat 1/50,000 - 1/100,000 dilution

    Predicted band size : 82 kDa
    Observed band size : 82 kDa
    Additional bands at : 46 kDa. We are unsure as to the identity of these extra bands.

References

ab66372 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab66372.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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