Overview

  • Product name
  • Description
    Rabbit polyclonal to ZNF229
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to amino acids 231-342 of Human ZNF229 (Q9UJW7).

  • Positive control
    • Human liver tissue and Human RT-4, U-251 MG, A-431, Liver and Tonsil cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • Function
    May be involved in transcriptional regulation.
  • Sequence similarities
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 18 C2H2-type zinc fingers.
    Contains 1 KRAB domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Zinc finger protein 229 antibody
    • ZN229_HUMAN antibody
    • ZNF229 antibody

Images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in vesicles. Recommended concentration of ab121139 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Immunohistochemical staining of paraffin embedded human liver tissue with anti-ZNF229 antibody (ab121139; dilution 1:150) showing cytoplasmic positivity in hepatocytes.

References

ab121139 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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