The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 42 kDa).
1/10. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Transcription factor required for myelination of differentiated oligodendrocytes. Required for the conversion of oligodendrocytes from the premyelinating to the myelinating state. In the developing central nervous system (CNS), involved in the maintenance in the progenitor stage by promoting the cell cycle. Specifically binds to the 5'-TCAT-3' DNA sequence (By similarity). Has transcription repressor activity in vitro.
Expressed in many tissues except in heart.
Belongs to the krueppel C2H2-type zinc-finger protein family. Contains 4 C2H2-type zinc fingers. Contains 1 SCAN box domain.
Flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling ZNF24 with ab180121 at 1/10 dilution (red) or a rabbit IgG (negative) (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/75 dilution. Isotype control Rabbit IgG.
Western blot - Anti-ZNF24 [EPR13975] antibody (ab180121)
All lanes : Anti-ZNF24 antibody [EPR13975] (ab180121) at 1/10000 dilution
Lane 1 : Jurkat cell lysate Lane 2 : HeLa cell lysate Lane 3 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution