Overview

  • Product nameAnti-ZNF258 antibody
  • Description
    Rabbit polyclonal to ZNF258
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Dog
  • Immunogen

    Synthetic peptide derived from residues 625 - 674 of human ZNF258.

    .

  • Positive control
    • WB: Jurkat cell lysate. IHC-P: Human kidney tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody is purified by peptide affinity chromatography method.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab33004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/62500.
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 79 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

Target

Anti-ZNF258 antibody images

  • Anti-ZNF258 antibody (ab33004) at 0.5 µg/ml + Jurkat cell lysate

    Secondary
    HRP conjugated anti Rabbit IgG 1/50000 - 1/100000

    Predicted band size : 79 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling ZNF258 at 4-8µg/ml. Cells with positive label (indicated by arrows) are epithelial cells of renal tubule. Magnification: 400X.

References for Anti-ZNF258 antibody (ab33004)

ab33004 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab33004.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"