The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.25 µg/ml. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
ELISA titre using peptide based assay 1:62500.
FunctionMay be involved in transcriptional regulation.
Tissue specificityRelatively highly expressed in kidney, thymus, testis, ovary, brain, lung, placenta, and prostate, and relatively low expression in heart, liver, skeletal muscle, pancreas, spleen, and small intestine.
Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family. Contains 13 C2H2-type zinc fingers. Contains 1 KRAB domain.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.