Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab33005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1.5625e+006.
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 62 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionMay be involved in transcriptional regulation.
  • Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 14 C2H2-type zinc fingers.
    Contains 1 KRAB domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • HZF1 antibody
    • KIAA2007 antibody
    • Zinc finger protein 1 antibody
    • Zinc finger protein 266 antibody
    • Zinc finger protein HZF1 antibody
    • ZN266_HUMAN antibody
    • ZNF 266 antibody
    • ZNF266 antibody
    see all

Anti-ZNF266 antibody images

  • Anti-ZNF266 antibody (ab33005) at 0.5 µg/ml + HepG2 cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG 1: 50,000 - 100,000

    Predicted band size : 62 kDa
    Observed band size : 62 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebellum tissue labelling ZNF266 with ab33005 at 4-8µg/ml. Arrows indicate positively labelled purkinje cells.

  • Anti-ZNF266 antibody (ab33005) at 1/1000 dilution + Lysates were prepared from human HepG2 cells at 25 µg

    Secondary
    HRP-conjugated goat polyclonal to rabbit IgG at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 62 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes

    This image is a courtesy of Seth Frietze

    See Abreview

References for Anti-ZNF266 antibody (ab33005)

ab33005 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - nuclear (HepG2 cells)
Loading amount 25 µg
Specification HepG2 cells
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5%
Username

Dr. Seth Frietze

Verified customer

Submitted Mar 04 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"