The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2.5 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 76 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
RelevanceZNF271 belongs to the krueppel C2H2-type zinc-finger protein family and contains 19 C2H2-type zinc fingers. Two named isoforms are produced by alternative splicing. It plays a role in the EBV-mediated transformation process and may be involved in transcriptional regulation. ZNF271 is expressed in T-cell lines, thymocytes, thyroid, and pancreatic islet cells following induction by Epstein-Barr virus.