A region within synthetic peptide: EVVEVLTLNQ EVAGPRNAQI QALYAEDGSL SADAPSEQVQ QQGKHPGDPE, corresponding to N terminal amino acids 109-158 of Human ZNF274
Our Abpromise guarantee covers the use of ab55913 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|WB||Use a concentration of 2.5 µg/ml. Detects a band of approximately 61 kDa (predicted molecular weight: 74 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use at an assay dependent concentration.|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human heart tissue labelling ZNF274 at 4-8µg/ml. Arrows indicate positively labelled myocardial cells. Magnification: 400X.
ChIP analysis using unpurified ab55913 binding ZNF274 antibody in nuclear lysate derived from K562 erythroleukemia human cell line. Cells were cross-linked for 10 minutes with 1% formaldehyde. 30ug of chromatin/sample was incubated with 4ug of undiluted primary antibody for 24 hours at 4°C. Protein binding was detected using real-time PCR.
Positive control: ZNF180, ZNF268, ZNF544
Negative Control: ZNF358 / ZNF274 KO