Overview

  • Product name
  • Description
    Rabbit polyclonal to ZNF280D
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Dog, Turkey, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Orangutan, Bat
  • Immunogen

    Synthetic peptide, corresponding to a region between residue 879 and 929 of Human ZNF280D (NP_060131.2).

  • Positive control
    • HeLa, 293T and Jurkat whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab114972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 109 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    May function as a transcription factor.
  • Sequence similarities
    Contains 5 C2H2-type zinc fingers.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • MGC21637 antibody
    • MGC61687 antibody
    • SUHW4 antibody
    • Suppressor of hairy wing homolog 4 (Drosophila) antibody
    • Suppressor of hairy wing homolog 4 antibody
    • Z280D_HUMAN antibody
    • Zinc finger protein 280D antibody
    • Zinc finger protein 634 antibody
    • Znf280d antibody
    • ZNF634 antibody
    see all

Images

  • All lanes : Anti-ZNF280D antibody (ab114972) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 109 kDa


    Exposure time : 30 seconds
  • Detection of ZNF280D in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab114972 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 10 seconds.

References

ab114972 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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