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Rabbit polyclonal to ZNF280D
Predicted to work with:
Chimpanzee, Gorilla, Orangutan
Synthetic peptide (Human) corresponding to a region between residue 929 and 979 of ZNF280D (NP_060131.2).
HeLa Whole cell lysate, 293T Whole cell lysate and Jurkat Whole cell lysate.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate Note: pH 7 to 8
Concentration information loading...
Immunogen affinity purified
Antibody was affinity purified using an epitope specific to ZNF280D immobilized on solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 109 kDa.
Use at 2-10 µg/mg of lysate.
May function as a transcription factor.
Contains 5 C2H2-type zinc fingers.
Information by UniProt
Western blot - Anti-ZNF280D antibody (ab115174)
All lanes : Anti-ZNF280D antibody (ab115174) at 0.1 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : 293T whole cell lysate at 50 µg Lane 4 : Jurkat whole cell lysate at 50 µg Developed using the ECL technique Predicted band size : 109 kDa Exposure time : 30 seconds
Immunoprecipitation - Anti-ZNF280D antibody (ab115174)
Detection of Human ZNF280D by Immunoprecipitation. Samples: Whole cell lysate from HeLa (1 mg for IP, 20% of IP loaded). ab115174 was used for IP at 6 ug/mg of lysate, and for subsequente WB detection, was used at 1 ug/ml.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"