The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1:312500.
Use a concentration of 1.25 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 62 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionMay be involved in the transcriptional activation of MDM2 and EP300 genes.
Tissue specificityExpressed in adult heart, brain, placenta, lung and kidney, but not in adult liver and skeletal muscle. In 17-day old embryo, detected in liver, skeletal muscle, brain, heart and small intestine.
Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family. Contains 7 C2H2-type zinc fingers. Contains 1 KRAB domain. Contains 1 SCAN box domain.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.