Overview

  • Product nameAnti-ZNF312 antibody
    See all ZNF312 primary antibodies
  • Description
    Rabbit polyclonal to ZNF312
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    Synthetic peptide: ASSASLETMV PPACPRAGAS PATSKTLAFS IERIMAKTSE PRAPFEPRPG, corresponding to amino acids 1-50 of Human ZNF312

  • Positive control
    • Jurkat cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab49245 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 49 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevanceZNF312, also known as Fezf2 or Fez-like, is a zinc finger protein that acts as a transcriptional repressor during the development of corticospinal motor neurons and other subcerebral projection neurons. ZNF312 is expressed by early progenitor cells in the ventricular zone. It regulates the fate choice of subcortical projection neurons in the developing cerebral cortex. This protein is expressed in the developing cortical plate during early embryonic development. During late embryonic development and early postnatal development, ZNF312 expression disappears from the cortical progenitors and becomes restricted to the subplate nad the prospective layer V and VI pyramidal neurons.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • Fez antibody
    • FEZ family zinc finger protein 2 antibody
    • FEZF 2 antibody
    • FEZF2 antibody
    • FEZL antibody
    • Forebrain embryonic zinc finger like protein 2 antibody
    • TOF antibody
    • ZFP 312 antibody
    • ZFP312 antibody
    • Zinc finger FEZL antibody
    • Zinc finger protein 312 antibody
    • Zinc finger protein FEZ like antibody
    • ZNF 312 antibody
    see all

Anti-ZNF312 antibody images

  • Lane 0 : Marker
    Lane 1 : Anti-ZNF312 antibody (ab49245) at 2.5 µg/ml

    Lane 0 : As above
    Lane 1 : Jurkat cell lysate at 10 µg with 5% skim milk in PBS.

    Secondary
    Lane 1 : HRP conjugated anti-rabbit IgG at 1/50000 dilution


    Predicted band size : 49 kDa
    Observed band size : 47 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-ZNF312 antibody (ab49245) at 2.5 µg/ml

    Lane 1 : MW Ladder
    Lane 2 : Cell lysates prepared from human Jurkat cells


    Predicted band size : 49 kDa

References for Anti-ZNF312 antibody (ab49245)

ab49245 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab49245.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"