Overview

  • Product nameAnti-ZNF312 antibody
    See all ZNF312 primary antibodies
  • Description
    Rabbit polyclonal to ZNF312
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Cow, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Mouse ZNF312.

  • Positive control
    • This antibody gave a positive signal in Mouse spinal cord tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab69436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).

Target

  • RelevanceZNF312, also known as Fezf2 or Fez-like, is a zinc finger protein that acts as a transcriptional repressor during the development of corticospinal motor neurons and other subcerebral projection neurons. ZNF312 is expressed by early progenitor cells in the ventricular zone. It regulates the fate choice of subcortical projection neurons in the developing cerebral cortex. This protein is expressed in the developing cortical plate during early embryonic development. During late embryonic development and early postnatal development, ZNF312 expression disappears from the cortical progenitors and becomes restricted to the subplate nad the prospective layer V and VI pyramidal neurons.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • Fez antibody
    • FEZ family zinc finger protein 2 antibody
    • FEZF 2 antibody
    • FEZF2 antibody
    • FEZL antibody
    • Forebrain embryonic zinc finger like protein 2 antibody
    • TOF antibody
    • ZFP 312 antibody
    • ZFP312 antibody
    • Zinc finger FEZL antibody
    • Zinc finger protein 312 antibody
    • Zinc finger protein FEZ like antibody
    • ZNF 312 antibody
    see all

Anti-ZNF312 antibody images

  • Anti-ZNF312 antibody (ab69436) at 1 µg/ml + Spinal Cord (Mouse) Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 48 kDa
    Observed band size : 48 kDa
    Additional bands at : 17 kDa,33 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
  • ICC/IF image of ab69436 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab69436, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-ZNF312 antibody (ab69436)

This product has been referenced in:
  • Shu XS  et al. FEZF2, a novel 3p14 tumor suppressor gene, represses oncogene EZH2 and MDM2 expression and is frequently methylated in nasopharyngeal carcinoma. Carcinogenesis 34:1984-93 (2013). WB . Read more (PubMed: 23677067) »

See 1 Publication for this product

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Induced Pluripotent Stem cell derived glutamatergi)
Gel Running Conditions Reduced Denaturing (Bolt 4-12% Bis-Tris Plus Gels)
Loading amount 4 µg
Specification Induced Pluripotent Stem cell derived glutamatergi
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Erika Yeh

Verified customer

Submitted Jun 26 2015

Application Immunocytochemistry
Sample Human Cultured Cells (Induced Pluripotent Stem cell derived neurons)
Permeabilization Yes - 90% Methanol
Specification Induced Pluripotent Stem cell derived neurons
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Dr. Erika Yeh

Verified customer

Submitted Jun 19 2015


I would be happy to offer you another code since you weren't able to use the original code. Our AbTrial program has changed though, so the code is worth the price you paid for the antibody, in this case $337. You use the code in the same way thoug...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thank you for submitting an Abreview for ab69436. As you may have noticed, your review has now been published on our website.

Since you obtained poor results using the antibody in an untested application, we would like to follow up on this t...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (brain)
Specification brain
Fixative 70% Ethanol
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH6 or high pH citrate buffer
Permeabilization Yes - blocking buffer containing 0.3% Triton X-100
Blocking step Superblock (thermo) as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 01 2012

DISCOUNT CODE: *
Expiration date: *

I am very pleased to hear you would like to accept our offer and test ab69436 in IHC-P. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an...

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Thank you very much for your interest in ab69436.

To our knowledge, ab69436 has not been tested in IHC-P. Therefore, I can offer a discount off a future purchase if you buy ab69436 now, test it inIHC-P and submit feedback to us in the form of ...

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Thank you for contacting Abcam earlier today.

I am sorry about the issues you have been having with ab18465 in IHC-FrFl. as we discussed, I am sending you a new vial of this antibody, from a different lot to test out.

As for ab69436...

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