The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml.
Use a concentration of 1 µg/ml. Predicted molecular weight: 52 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionTranscription repressor. Involved in the axonal projection and proper termination of olfactory sensory neurons (OSN). Plays a role in rostro-caudal patterning of the diencephalon and in prethalamic formation. Expression is required in OSN to cell-autonomously regulate OSN axon projections. Regulates non-cell-autonomously the layer formation of the olfactory bulb development and the interneurons. May be required for correct rostral migration of the interneuron progenitors.
Tissue specificityExpressed in brain. Little or no expression in other tissues. Overexpresssed specifically in gastic cancers. A 2-to 20-fold increase is found in over 50% of gastric cancer tissues.
Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family. Contains 6 C2H2-type zinc fingers.