Anti-RNF114 antibody (ab97303)
Key features and details
- Rabbit polyclonal to RNF114
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-RNF114 antibody -
Description
Rabbit polyclonal to RNF114 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig -
Immunogen
Recombinant fragment corresponding to Human RNF114 aa 1-220.
-
Positive control
- WB: 293T, A431, H1299, HeLaS3, HepG2, MOLT4 or Raji cell lysate; ICC/IF: HeLa cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97303 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 26 kDa.
|
|
ICC/IF |
1/100 - 1/200.
|
Notes |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 26 kDa. |
ICC/IF
1/100 - 1/200. |
Target
-
Function
May play a role in spermatogenesis. -
Tissue specificity
Expressed in numerous tissues, including skin, CD4 lymphocytes and dendritic cells. Highest levels in testis. -
Sequence similarities
Contains 1 RING-type zinc finger. - Information by UniProt
-
Database links
- Entrez Gene: 513479 Cow
- Entrez Gene: 55905 Human
- Entrez Gene: 81018 Mouse
- Entrez Gene: 414917 Pig
- Entrez Gene: 362277 Rat
- Omim: 612451 Human
- SwissProt: Q4U5R4 Cow
- SwissProt: Q9Y508 Human
see all -
Alternative names
- 1110008J21Rik antibody
- AI225886 antibody
- AW549494 antibody
see all
Images
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab97303 has been referenced in 2 publications.
- Zhang X et al. Extracellular fibrinogen-binding protein released by intracellular Staphylococcus aureus suppresses host immunity by targeting TRAF3. Nat Commun 13:5493 (2022). PubMed: 36123338
- Bai Y et al. Proteasomal degradation of nonstructural protein 12 by RNF114 suppresses porcine reproductive and respiratory syndrome virus replication. Vet Microbiol 246:108746 (2020). PubMed: 32605740