Overview

  • Product name
  • Description
    Mouse polyclonal to ZNF331
  • Specificity
    This antibody is reactive against mammalian transfected lysate.
  • Tested applications
    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    ZNF331 (AAH09433.1, 1 a.a. ~ 463 a.a) full-length human protein.

  • Positive control
    • ZNF331 transfected 293T cell lysate and Hela cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab67886 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 22370639
ICC/IF Use a concentration of 10 µg/ml. NOTE: Product requires purification with Protein A prior to use in ICC/IF.
WB 1/500 - 1/1000. Detects a band of approximately 44 kDa (predicted molecular weight: 54 kDa).

Target

  • Function
    May be involved in transcriptional regulation. May play a role in spermatogenesis.
  • Tissue specificity
    Testis specific.
  • Sequence similarities
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 12 C2H2-type zinc fingers.
    Contains 1 KRAB domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • C2H2 like zinc finger protein antibody
    • C2H2 like zinc finger protein rearranged in thyroid adenomas antibody
    • C2H2-like zinc finger protein rearranged in thyroid adenomas antibody
    • DKFZp686L0787 antibody
    • KRAB zinc finger protein antibody
    • OTTHUMP00000223038 antibody
    • OTTHUMP00000223039 antibody
    • OTTHUMP00000223040 antibody
    • OTTHUMP00000223041 antibody
    • OTTHUMP00000223042 antibody
    • OTTHUMP00000223043 antibody
    • OTTHUMP00000223045 antibody
    • OTTHUMP00000223046 antibody
    • OTTHUMP00000223047 antibody
    • OTTHUMP00000223048 antibody
    • OTTHUMP00000223052 antibody
    • OTTHUMP00000223053 antibody
    • OTTHUMP00000223054 antibody
    • RITA antibody
    • Zinc finger protein 331 antibody
    • Zinc finger protein 361 antibody
    • Zinc finger protein 463 antibody
    • ZN331_HUMAN antibody
    • ZNF331 antibody
    • ZNF361 antibody
    • ZNF463 antibody
    see all

Images

  • All lanes : Anti-ZNF331 antibody (ab67886) at 1/500 dilution

    Lane 1 : ZNF331 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 54 kDa
    Observed band size : 44 kDa (why is the actual band size different from the predicted?)
  • ab67886 at 10µg/ml staining ZNF331 in Hela cells.

References

This product has been referenced in:
  • Yu J  et al. Zinc-finger protein 331, a novel putative tumor suppressor, suppresses growth and invasiveness of gastric cancer. Oncogene : (2012). WB, IHC-P ; Human . Read more (PubMed: 22370639) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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