Overview

  • Product nameAnti-ZNF342 antibody
    See all ZNF342 primary antibodies
  • Description
    Rabbit polyclonal to ZNF342
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (Human)

  • Positive control
    • Jurkat cell lysate, Human kidney and stomach tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab51265 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.125 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

Anti-ZNF342 antibody images

  • Lane 1 : Molecular weight markers
    Lane 2 : Anti-ZNF342 antibody (ab51265) at 0.125 µg/ml

    Lane 1 : As above
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 50 kDa
    Observed band size : 50 kDa
  • Ab51265 at 8 µg/ml staining epithelial cells of renal tubules from paraffin embedded human kidney.
  • Ab51265 at 8 µg/ml staining epithelial cells of fundic gland from paraffin embedded human stomach.

References for Anti-ZNF342 antibody (ab51265)

This product has been referenced in:
  • Levin VA  et al. Different changes in protein and phosphoprotein levels result from serum starvation of high-grade glioma and adenocarcinoma cell lines. J Proteome Res 9:179-91 (2010). WB ; Human . Read more (PubMed: 19894763) »
  • Poland KS  et al. Overexpression of ZNF342 by juxtaposition with MPO promoter/enhancer in the novel translocation t(17;19)(q23;q13.32) in pediatric acute myeloid leukemia and analysis of ZNF342 expression in leukemia. Genes Chromosomes Cancer 48:480-9 (2009). Read more (PubMed: 19255975) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - nuclear (Multiple cell types)
Loading amount 30 µg
Specification Multiple cell types
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Seth Frietze

Verified customer

Submitted Jun 30 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"