Overview

  • Product name
    Anti-ZNF345 antibody
  • Description
    Rabbit polyclonal to ZNF345
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Monkey
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 1-199 of Human ZNF345.

  • Positive control
    • NT2D1, IMR32 and MCF7 cell lysates; MCF7 cells; Human BT474 xenograft tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78% PBS, 20% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab153921 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Predicted molecular weight: 55 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

  • Function
    May be involved in transcriptional regulation.
  • Sequence similarities
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 15 C2H2-type zinc fingers.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ36827 antibody
    • FLJ51247 antibody
    • FLJ96023 antibody
    • HZF10 antibody
    • MGC33794 antibody
    • OTTHUMP00000232107 antibody
    • OTTHUMP00000232108 antibody
    • OTTHUMP00000232110 antibody
    • Zinc finger protein 345 antibody
    • Zinc finger protein HZF10 antibody
    • ZN345_HUMAN antibody
    • ZNF345 antibody
    see all

Images

  • Anti-ZNF345 antibody (ab153921) at 1/10000 dilution + MCF7 whole cell lysate at 30 µg

    Predicted band size: 55 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded BT474 xenograft labeling ZNF345 with ab153921 at 1/100 dilution.
  • Immunofluorescent analysis of paraformaldehyde-fixed MCF7 cells labeling ZNF345 with ab153921 at 1/500 dilution. Lower panel co-stained with Hoechst 33342.

References

ab153921 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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