Overview

  • Product name
  • Description
    Rabbit polyclonal to ZNF350
  • Tested applications
    Suitable for: WB, ChIPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic 20 amino acid peptide from near the carboxy terminus of human ZNF350 (NP_067645).

  • Positive control
    • A-20 lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab77085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 60 kDa.Can be blocked with ZNF350 peptide (ab150315).
ChIP Use at an assay dependent dilution. PubMed: 20926453

Target

  • Function
    Transcriptional repressor. Binds to a specific sequence, 5'-GGGxxxCAGxxxTTT-3', within GADD45 intron 3.
  • Tissue specificity
    Widely expressed.
  • Sequence similarities
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 8 C2H2-type zinc fingers.
    Contains 1 KRAB domain.
  • Cellular localization
    Nucleus matrix. Associated with the nuclear matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • KRAB zinc finger protein ZFQR antibody
    • ZBRK1 antibody
    • ZFQR antibody
    • Zinc finger and BRCA1 interacting protein with a KRAB domain 1 antibody
    • Zinc finger and BRCA1-interacting protein with a KRAB domain 1 antibody
    • Zinc finger protein 350 antibody
    • Zinc finger protein ZBRK1 antibody
    • ZN350_HUMAN antibody
    • ZNF350 antibody
    see all

Anti-ZNF350 antibody images

  • All lanes : Anti-ZNF350 antibody (ab77085) at 1 µg/ml

    Lane 1 : A-20 lysate
    Lane 2 : A-20 lysate with blocking peptide

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 60 kDa
    Observed band size : 60 kDa

References for Anti-ZNF350 antibody (ab77085)

This product has been referenced in:
  • Calderon MR  et al. Ligand-dependent corepressor contributes to transcriptional repression by C2H2 zinc-finger transcription factor ZBRK1 through association with KRAB-associated protein-1. Nucleic Acids Res 42:7012-27 (2014). ChIP . Read more (PubMed: 24829459) »
  • Hallen L  et al. The KRAB-containing zinc-finger transcriptional regulator ZBRK1 activates SCA2 gene transcription through direct interaction with its gene product, ataxin-2. Hum Mol Genet : (2010). WB, ChIP ; Human . Read more (PubMed: 20926453) »

See all 2 Publications for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Monkey Cell (Kiney)
Permeabilization
Yes - 0.1% troton x-100
Specification
Kiney
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 14 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Monkey Cell lysate - whole cell (Kidney)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (4-12)
Loading amount
30 µg
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
Username

Abcam user community

Verified customer

Submitted Apr 08 2016

Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
30 µg
Specification
Brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Apr 08 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Feb 23 2016

Thank you for contacting us.

Following our email correspondance last week, I am pleased to inform you that we have added the ZNF350 synthetic 20 amino acid peptide to our catalogue with reference number ab150315. You can find it on our websi...

Read More

Thank you for contacting us and your interest in our products.

The immunogen sequence used to raise the anti-ZNF350 antibody (ab77085) is proprietary information. However, in order to aid your research I am able to share it with you. Please do...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (colon, ovary)
Loading amount
20 µg
Specification
colon, ovary
Gel Running Conditions
Reduced Denaturing (12 % gel)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Sep 14 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up