Anti-ZNF350 antibody - ChIP Grade (ab77085)

Overview

  • Product nameAnti-ZNF350 antibody - ChIP Grade
    See all ZNF350 primary antibodies
  • Description
    Rabbit polyclonal to ZNF350 - ChIP Grade
  • Tested applicationsSuitable for: WB, ChIPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic 20 amino acid peptide from near the carboxy terminus of human ZNF350 (NP_067645).

  • Positive control
    • A-20 lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab77085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 60 kDa.Can be blocked with ZNF350 peptide (ab150315).
ChIP Use at an assay dependent dilution. PubMed: 20926453

Target

  • FunctionTranscriptional repressor. Binds to a specific sequence, 5'-GGGxxxCAGxxxTTT-3', within GADD45 intron 3.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 8 C2H2-type zinc fingers.
    Contains 1 KRAB domain.
  • Cellular localizationNucleus matrix. Associated with the nuclear matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • KRAB zinc finger protein ZFQR antibody
    • ZBRK1 antibody
    • ZFQR antibody
    • Zinc finger and BRCA1 interacting protein with a KRAB domain 1 antibody
    • Zinc finger and BRCA1-interacting protein with a KRAB domain 1 antibody
    • Zinc finger protein 350 antibody
    • Zinc finger protein ZBRK1 antibody
    • ZN350_HUMAN antibody
    • ZNF350 antibody
    see all

Anti-ZNF350 antibody - ChIP Grade images

  • All lanes : Anti-ZNF350 antibody - ChIP Grade (ab77085) at 1 µg/ml

    Lane 1 : A-20 lysate
    Lane 2 : A-20 lysate with blocking peptide

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 60 kDa
    Observed band size : 60 kDa

References for Anti-ZNF350 antibody - ChIP Grade (ab77085)

This product has been referenced in:
  • Calderon MR  et al. Ligand-dependent corepressor contributes to transcriptional repression by C2H2 zinc-finger transcription factor ZBRK1 through association with KRAB-associated protein-1. Nucleic Acids Res 42:7012-27 (2014). ChIP . Read more (PubMed: 24829459) »
  • Hallen L  et al. The KRAB-containing zinc-finger transcriptional regulator ZBRK1 activates SCA2 gene transcription through direct interaction with its gene product, ataxin-2. Hum Mol Genet : (2010). WB, ChIP ; Human . Read more (PubMed: 20926453) »

See all 2 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Monkey Cell (Kiney)
Permeabilization Yes - 0.1% troton x-100
Specification Kiney
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative Paraformaldehyde
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Submitted Jun 14 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Monkey Cell lysate - whole cell (Kidney)
Gel Running Conditions Non-reduced Non-Denaturing (Native) (4-12)
Loading amount 30 µg
Specification Kidney
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
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Submitted Apr 08 2016

Application Western blot
Sample Mouse Tissue lysate - whole (Brain)
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Loading amount 30 µg
Specification Brain
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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Submitted Apr 08 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain)
Permeabilization No
Specification Brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative Paraformaldehyde
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Verified customer

Submitted Feb 23 2016

Thank you for contacting us.

Following our email correspondance last week, I am pleased to inform you that we have added the ZNF350 synthetic 20 amino acid peptide to our catalogue with reference number ab150315. You can find it on our websi...

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Thank you for contacting us and your interest in our products.

The immunogen sequence used to raise the anti-ZNF350 antibody (ab77085) is proprietary information. However, in order to aid your research I am able to share it with you. Please do...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (colon, ovary)
Loading amount 20 µg
Specification colon, ovary
Gel Running Conditions Reduced Denaturing (12 % gel)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
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Verified customer

Submitted Sep 14 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"