The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesPeptide ELISA: Antibody detection limit dilution 1:32,000.
WB: Use at a concentration of 0.3 - 1.0 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionActs as an E2-dependent E3 ubiquitin-protein ligase. May be involved in endocytic trafficking.
Tissue specificityExpressed at extremely low levels in normal breast, prostate, lung, colon. Higher levels of expression are detected in heart, skeletal muscle, testis as well as in breast and prostate cancer cells.
PathwayProtein modification; protein ubiquitination.