Overview

  • Product name
  • Description
    Rabbit polyclonal to ZNF364
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide derived from a region encoded by C terminal amino acids 254-303 (IVPWLELHDT CPVCRKSLNG EDSTRQSQST EASASNRFSN DSQLHDRWTF) of Human ZNF364

  • Positive control
    • HepG2 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab80432 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 34 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Acts as an E2-dependent E3 ubiquitin-protein ligase. May be involved in endocytic trafficking.
  • Tissue specificity
    Expressed at extremely low levels in normal breast, prostate, lung, colon. Higher levels of expression are detected in heart, skeletal muscle, testis as well as in breast and prostate cancer cells.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Contains 1 RING-type zinc finger.
  • Post-translational
    modifications
    RING-type zinc finger-dependent and E2-dependent autoubiquitination.
  • Cellular localization
    Cytoplasm > cytosol. The GTP-bound form of RAB7A recruits RNF115 from the cytosol onto late endosomes/lysosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • BCA2 antibody
    • Breast cancer associated gene 2 antibody
    • CL469780 antibody
    • E3 ubiquitin-protein ligase RNF115 antibody
    • Rabring 7 antibody
    • RING finger protein 115 antibody
    • RN115_HUMAN antibody
    • RNF115 antibody
    • Zinc finger protein 364 antibody
    see all

Images

  • Anti-ZNF364 antibody (ab80432) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 34 kDa
    Observed band size : 34 kDa

References

This product has been referenced in:
  • Nityanandam R & Serra-Moreno R BCA2/Rabring7 targets HIV-1 Gag for lysosomal degradation in a tetherin-independent manner. PLoS Pathog 10:e1004151 (2014). WB . Read more (PubMed: 24852021) »
  • Rodrigo-Brenni MC  et al. Cytosolic quality control of mislocalized proteins requires RNF126 recruitment to Bag6. Mol Cell 55:227-37 (2014). Human . Read more (PubMed: 24981174) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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