The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1.25 µg/ml. Detects a band of approximately 59 kDa (predicted molecular weight: 64 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/1562500.
RelevanceZNF382 is a member of the C2H2 zinc finger transcription factor family, which plays key roles in the regulation of cell proliferation, differentiation, and apoptosis in response to a variety of stimuli.