Overview

  • Product name
  • Description
    Rabbit polyclonal to ZNF423
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Recombinant protein fragment, corresponding to a region within amino acids 250-300 of Human ZNF423 (NP_055884.2, Uniprot: Q2M1K9)

  • Positive control
    • 293T cell lysate.

Applications

Our Abpromise guarantee covers the use of ab169096 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 145 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    Transcription factor that can both act as an activator or a repressor depending on the context. Plays a central role in BMP signaling and olfactory neurogenesis. Associates with SMADs in response to BMP2 leading to activate transcription of BMP target genes. Acts as a transcriptional repressor via its interaction with EBF1, a transcription factor involved in terminal olfactory receptor neurons differentiation; this interaction preventing EBF1 to bind DNA and activate olfactory-specific genes. Involved in olfactory neurogenesis by participating in a developmental switch that regulates the transition from differentiation to maturation in olfactory receptor neurons. Controls proliferation and differentiation of neural precursors in cerebellar vermis formation.
  • Tissue specificity
    Expressed in brain, lung, skeletal muscle, heart, pancreas and kidney but not liver or placenta. Also expressed in aorta, ovary, pituitary, small intestine, fetal brain, fetal kidney and, within the adult brain, in the substantia nigra, medulla, amygdala, thalamus and cerebellum.
  • Sequence similarities
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 30 C2H2-type zinc fingers.
  • Domain
    Uses different DNA- and protein-binding zinc fingers to regulate the distinct BMP-Smad and Olf signaling pathways. C2H2-type zinc fingers 14-19 mediate the interaction with SMAD1 and SMAD4, while zinc fingers 28-30 mediate the interaction with EBF1. zinc fingers 2-8 bind the 5'-CCGCCC-3' DNA sequence in concert with EBF1, while zinc fingers 9-13 bind BMP target gene promoters in concert with SMADs.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Early B cell factor associated zinc finger protein antibody
    • Ebfaz antibody
    • hOAZ antibody
    • JBTS19 antibody
    • KIAA0760 antibody
    • NPHP14 antibody
    • Nur12 antibody
    • OAZ antibody
    • OLF 1/EBF associated zinc finger antibody
    • OLF1/EBF associated zinc finger protein antibody
    • Olf1/EBF-associated zinc finger protein antibody
    • Roaz antibody
    • Smad and Olf interacting zinc finger protein antibody
    • Smad- and Olf-interacting zinc finger protein antibody
    • Zfp104 antibody
    • ZFP423 antibody
    • zinc finger protein 423, mouse, homolog of antibody
    • Zinc finger protein 423 antibody
    • ZN423_HUMAN antibody
    • ZNF423 antibody
    • ZNF423 zinc finger protein 423 antibody
    see all

Images

  • All lanes : Anti-ZNF423 antibody (ab169096) at 0.4 µg/ml

    Lane 1 : 293T cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 145 kDa


    Exposure time: 3 minutes
  • Immunoprecipitation analysis of 1 mg (20% of IP loaded) of 293T whole cell lysate using: 1) ab169096 (6 µg/mg lysate), 2) another rabbit anti-ZNF423 antibody and 3) control IgG. For blotting ab169096 was used at 1 µg/ml. Detection by chemiluminescence with an exposure time of 3 minutes.

References

ab169096 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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