Overview

  • Product nameAnti-ZNF423 antibody
    See all ZNF423 primary antibodies
  • Description
    Rabbit polyclonal to ZNF423
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 1235 - 1284 (FTVFVQANKL QQHIFAVHGQ EDKIYDCSQC PQKFFFQTEL QNHTMSQHAQ) of Human ZNF423 (NP_055884).

  • Positive control
    • HT1080 cell lysate

Applications

Our Abpromise guarantee covers the use of ab94451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 145 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionTranscription factor that can both act as an activator or a repressor depending on the context. Plays a central role in BMP signaling and olfactory neurogenesis. Associates with SMADs in response to BMP2 leading to activate transcription of BMP target genes. Acts as a transcriptional repressor via its interaction with EBF1, a transcription factor involved in terminal olfactory receptor neurons differentiation; this interaction preventing EBF1 to bind DNA and activate olfactory-specific genes. Involved in olfactory neurogenesis by participating in a developmental switch that regulates the transition from differentiation to maturation in olfactory receptor neurons. Controls proliferation and differentiation of neural precursors in cerebellar vermis formation.
  • Tissue specificityExpressed in brain, lung, skeletal muscle, heart, pancreas and kidney but not liver or placenta. Also expressed in aorta, ovary, pituitary, small intestine, fetal brain, fetal kidney and, within the adult brain, in the substantia nigra, medulla, amygdala, thalamus and cerebellum.
  • Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 30 C2H2-type zinc fingers.
  • DomainUses different DNA- and protein-binding zinc fingers to regulate the distinct BMP-Smad and Olf signaling pathways. C2H2-type zinc fingers 14-19 mediate the interaction with SMAD1 and SMAD4, while zinc fingers 28-30 mediate the interaction with EBF1. zinc fingers 2-8 bind the 5'-CCGCCC-3' DNA sequence in concert with EBF1, while zinc fingers 9-13 bind BMP target gene promoters in concert with SMADs.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Early B cell factor associated zinc finger protein antibody
    • Ebfaz antibody
    • hOAZ antibody
    • JBTS19 antibody
    • KIAA0760 antibody
    • NPHP14 antibody
    • Nur12 antibody
    • OAZ antibody
    • OLF 1/EBF associated zinc finger antibody
    • OLF1/EBF associated zinc finger protein antibody
    • Olf1/EBF-associated zinc finger protein antibody
    • Roaz antibody
    • Smad and Olf interacting zinc finger protein antibody
    • Smad- and Olf-interacting zinc finger protein antibody
    • Zfp104 antibody
    • ZFP423 antibody
    • zinc finger protein 423, mouse, homolog of antibody
    • Zinc finger protein 423 antibody
    • ZN423_HUMAN antibody
    • ZNF423 antibody
    • ZNF423 zinc finger protein 423 antibody
    see all

Anti-ZNF423 antibody images

  • Anti-ZNF423 antibody (ab94451) at 1 µg/ml (in 5% skim milk / PBS buffer) + HT1080 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 145 kDa

References for Anti-ZNF423 antibody (ab94451)

ab94451 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (fibrosarcoma cells)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Specification fibrosarcoma cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Abcam user community

Verified customer

Submitted Aug 26 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (293FT overexpression FUW-ZNF423)
Specification 293FT overexpression FUW-ZNF423
Fixative Paraformaldehyde
Permeabilization Yes - 0.2% Triton-X100 and 0.1% TW20 in PBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 25 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"