Overview

  • Product nameAnti-ZNF426 antibody
    See all ZNF426 primary antibodies
  • Description
    Rabbit polyclonal to ZNF426
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    A region within synthetic peptide: VMLENYKNLA TVGGQIIKPS LISWLEQEES RTVQGGVLQG WEMRLETQWS, corresponding to N terminal amino acids 70-119 of Human ZNF426

  • Positive control
    • Fetal liver lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab30967 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/62500.
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 61 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Anti-ZNF426 antibody images

  • Anti-ZNF426 antibody (ab30967) at 0.5 µg/ml (in 5% skim milk / PBS) + Fetal liver lysate

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 61 kDa
    Observed band size : 57 kDa (why is the actual band size different from the predicted?)

References for Anti-ZNF426 antibody (ab30967)

ab30967 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - nuclear (Multiple cell types)
Loading amount 30 µg
Specification Multiple cell types
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Seth Frietze

Verified customer

Submitted Jul 01 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"