The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Titre using peptide based assay: 1/312500.
Use a concentration of 2.5 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 69 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
RelevanceZNF440 (Zinc finger protein 440) contains 1 KRAB domain and 12 C2H2-type zinc fingers. ZNF440 belongs to the krueppel C2H2-type zinc-finger protein family and may be involved in transcriptional regulation.