Overview

  • Product nameAnti-ZNF440 antibody
  • Description
    Rabbit polyclonal to ZNF440
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide: RNLTSLGKRW KDQNIEYEHQ NPRRNFRSLI EEKVNEIKDD SHCGETFTPV , corresponding to amino acids 38-87 of Human ZNF440

  • Positive control
    • HepG2 cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab66849 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 69 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevanceZNF440 (Zinc finger protein 440) contains 1 KRAB domain and 12 C2H2-type zinc fingers. ZNF440 belongs to the krueppel C2H2-type zinc-finger protein family and may be involved in transcriptional regulation.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • FLJ37933 antibody
    • Zinc finger protein 440 antibody
    • ZNF 440 antibody

Anti-ZNF440 antibody images

  • All lanes : Anti-ZNF440 antibody (ab66849) at 2.5 µg/ml (in 5% skim milk / PBS buffer)

    Lane 1 : Molecular weight markers
    Lane 2 : HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 69 kDa
    Observed band size : 58 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa,32 kDa,36 kDa. We are unsure as to the identity of these extra bands.

References for Anti-ZNF440 antibody (ab66849)

ab66849 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"